Why use this network to generate data?

Experiments using machine learning technologies can be performed to recognize advanced patterns of your output, and the network subsequently updates the pressures adaptively according to your experiment.

You can design your experimental models to test the effects of pressure to a living model iteratively.

The experimental design is a central focus of this site. With well-designed experiments that are very responsive, you can implement cycles of pressures that can cause multiple effects, especially if utilizing different frequencies.

Experimental Setup Experimental Controls Additional Experiments Rationale for Utilizing Deep-Learning

Drosophila larvae motility-displacement, calculate force/acceleration to sound pressure approximate location. (The API will create maps that can scale to the size of values for experiment)
Perform control measurements for each specimen studied and Examine perceived muscle resistance to sound pressure.
Examine how the drosophila accelerates after repeated exposure to increased force to impede movement.

Examine bidirectional resistance adaptation and acceleration by the larvae
Drop sound pressure (pulse-chase), and examine at what force the larvae push harder and at what point do the larvae start to accommodate with no sound pressure,then reapply sound pressure adaptively to see if the larvae can build a higher frequency and or amplitude of forces
Examine whether ECM or musculature is affected by sound pressures by iteratively applying sound, further comparing controls (no sound pressures) to the experimental group (sound pressures).
This experiment is based on the presumption that ECM structural changes will positively correlate to motility. By altered force transduction in the surrounding ECM structure, muscles contract in a particular way regardless of low to high levels of force exertion, as the muscles rely on the ECM to exert and extend the contraction.

Then examine the difference between the pressure (air) exerted by the outside exoskeleton to
For more complicated studies, create differential effects that fall into the logical constraints of your experimental design

Data can be collected and standardized specifically for neural network implementation, which is ancillary to molecular, genetic, behavioral, and modeling investigations. It is ancillary because deep neural networks derive patterns from a very randomized and stochastic process, it is not preferred to standard techniques in biological and biomedical research. However, a dynamic an open model that is highly adaptable and powerful may offer insight into translating dynamic effects like sound to the complexity and variation of many disease models. Also, computer-generated audible-pressure specifically fine-tuned to mediate molecular force signaling in tissue in itself is a very interesting question. Imagine an adaptable drug.

Experimental Uses

In Drosophila

◦ Embryology (Drosophila and other organisms) influence with a fine temporal resolution and application of pressure. (Outer effects have a more increased resolution (Air in sound))
◦ Wound healing experiments with Drosophila larvae.
◦ Neuronal experiments in combination with sensory to determine neural musculature signaling

Ex-vivo Experiments

◦ Applying force to explanted muscles, cardiac tissue, etc.
◦ Vasculature experiments (sprouting assays)
◦ Wound healing/Other Response experiments (ex-vivo tissue)
◦ Bioreactor Experiments

Possible Medical Use

if data generated is impactful with high throughput of network response and API/(neural network) performance

• Tissue Injury (Sports, Surgical, Accidental)
• Sports/Exercise Training
• Physical and Occupational Therapy
• Naturopathic Medicine
◦ Drug Delivery
◦ Transforming Active Cells,via precise for molecular effects (wide implications)

Basic Experimental Setup for Drosophila melanogaster (larvae)

Drosophila melanogaster has been extensively studied, genotyped; therefore molecular effects can be characterized considering a wealth of context and known biology. It also is a very small organism in which very little evasive experimentation can be utilized. Avoid using frequencies below 1000 Hz as that frequency is at the same frequency of predators(wasps).

**Is for experimental use only must safely operate audio technology according to the audio speaker/system technology and computer system.

In air: Testing the effects of air pressure on the exoskeleton and cuticle and its relation to larvae locomotion.

Make sure you have sufficient drosophila larvae melanogaster larvae possible at the 3rd instar.

Creating 3% agarose structure, dabbed with 15% sucrose solution. Wash larva with 15% sucrose solution, and observing for 5 minute periods with 2 seconds per frame.

Manually/automatically take pictures of the organism and upload it to the site. Follow directions to upload frame at the appropriate time. (Automation of this process for the user will implemented at a later time pending interest.)